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respective elisa kits  (Cusabio)


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    Cusabio respective elisa kits
    Fig. 4. Effect of GGD treatment on lung in flammatory and ECM deposition in mice induced by BLM. On day 14 and 28 after BLM instillation, lung tissues of mice were separated and stained with H&E (A, red arrow: lymphocytes, black arrow: neutro phils) and Masson (B) staining (magnifica tion 200x), respectively. The content <t>of</t> <t>hydroxyproline</t> in the lung tissues was measured by <t>ELISA</t> (C). Data were expressed as means ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Respective Elisa Kits, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/respective elisa kits/product/Cusabio
    Average 93 stars, based on 29 article reviews
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    Images

    1) Product Images from "Antifibrotic effect of Gancao Ganjiang decoction is mediated by PD-1 / TGF-β1 / IL-17A pathway in bleomycin-induced idiopathic pulmonary fibrosis."

    Article Title: Antifibrotic effect of Gancao Ganjiang decoction is mediated by PD-1 / TGF-β1 / IL-17A pathway in bleomycin-induced idiopathic pulmonary fibrosis.

    Journal: Journal of ethnopharmacology

    doi: 10.1016/j.jep.2021.114522

    Fig. 4. Effect of GGD treatment on lung in flammatory and ECM deposition in mice induced by BLM. On day 14 and 28 after BLM instillation, lung tissues of mice were separated and stained with H&E (A, red arrow: lymphocytes, black arrow: neutro phils) and Masson (B) staining (magnifica tion 200x), respectively. The content of hydroxyproline in the lung tissues was measured by ELISA (C). Data were expressed as means ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Fig. 4. Effect of GGD treatment on lung in flammatory and ECM deposition in mice induced by BLM. On day 14 and 28 after BLM instillation, lung tissues of mice were separated and stained with H&E (A, red arrow: lymphocytes, black arrow: neutro phils) and Masson (B) staining (magnifica tion 200x), respectively. The content of hydroxyproline in the lung tissues was measured by ELISA (C). Data were expressed as means ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Staining, Enzyme-linked Immunosorbent Assay, Control

    Fig. 7. Effect of pirfenidone and GGD on the content of IL-17A in the lung tissues of BLM-induced mice. The content of IL-17A was analyzed by IHC staining (magnification 100x and 200x) (A) and ELISA (B). Data were expressed as mean ± SD, n = 6. ##P ＜ 0.01 vs. control group. **P ＜ 0.01, *P ＜ 0.05 vs. BLM.
    Figure Legend Snippet: Fig. 7. Effect of pirfenidone and GGD on the content of IL-17A in the lung tissues of BLM-induced mice. The content of IL-17A was analyzed by IHC staining (magnification 100x and 200x) (A) and ELISA (B). Data were expressed as mean ± SD, n = 6. ##P ＜ 0.01 vs. control group. **P ＜ 0.01, *P ＜ 0.05 vs. BLM.

    Techniques Used: Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Control

    Fig. 8. Effect of pirfenidone and GGD on the content of TGF-β1 and mRNA/protein levels of EMT marker in the lung tissues. The content of TGF-β1 was detected by ELISA (A). The expression of E-cadherin, vimentin, and α-SMA was evaluated by qRT-PCR (B, C, D). The protein levels of E-cadherin, vimentin, and α-SMA were detected by Western blot (E). Data were expressed as mean ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM.
    Figure Legend Snippet: Fig. 8. Effect of pirfenidone and GGD on the content of TGF-β1 and mRNA/protein levels of EMT marker in the lung tissues. The content of TGF-β1 was detected by ELISA (A). The expression of E-cadherin, vimentin, and α-SMA was evaluated by qRT-PCR (B, C, D). The protein levels of E-cadherin, vimentin, and α-SMA were detected by Western blot (E). Data were expressed as mean ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM.

    Techniques Used: Marker, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot, Control



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    Changes of inflammatory cytokine expression in the Control, LPS, and LPS + QKPCG groups. (a) Relative expression of IL‐6, IL‐1α, IL‐1β, TNF‐α, <t>and</t> <t>TGF‐β</t> mRNA. (b) The concentration of IL‐1α, IL‐1β, TNF‐α, and TGF‐β in BALF, as determined via <t>ELISA.</t> (c) IL‐6 concentration in BALF and plasma, as determined via ELISA. ns p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. BALF, bronchoalveolar lavage fluid; ELISA, enzyme‐linked immunosorbent assay; LPS, lipopolysaccharide; QKPCG, Qingke Pingchuan granules; TGF‐β, transforming growth factor β; TNF‐α, tumor necrosis factor‐α.
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    Fig. 4. Effect of GGD treatment on lung in flammatory and ECM deposition in mice induced by BLM. On day 14 and 28 after BLM instillation, lung tissues of mice were separated and stained with H&E (A, red arrow: lymphocytes, black arrow: neutro phils) and Masson (B) staining (magnifica tion 200x), respectively. The content <t>of</t> <t>hydroxyproline</t> in the lung tissues was measured by <t>ELISA</t> (C). Data were expressed as means ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Fig. 4. Effect of GGD treatment on lung in flammatory and ECM deposition in mice induced by BLM. On day 14 and 28 after BLM instillation, lung tissues of mice were separated and stained with H&E (A, red arrow: lymphocytes, black arrow: neutro phils) and Masson (B) staining (magnifica tion 200x), respectively. The content <t>of</t> <t>hydroxyproline</t> in the lung tissues was measured by <t>ELISA</t> (C). Data were expressed as means ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    Changes of inflammatory cytokine expression in the Control, LPS, and LPS + QKPCG groups. (a) Relative expression of IL‐6, IL‐1α, IL‐1β, TNF‐α, and TGF‐β mRNA. (b) The concentration of IL‐1α, IL‐1β, TNF‐α, and TGF‐β in BALF, as determined via ELISA. (c) IL‐6 concentration in BALF and plasma, as determined via ELISA. ns p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. BALF, bronchoalveolar lavage fluid; ELISA, enzyme‐linked immunosorbent assay; LPS, lipopolysaccharide; QKPCG, Qingke Pingchuan granules; TGF‐β, transforming growth factor β; TNF‐α, tumor necrosis factor‐α.

    Journal: Pulmonary Circulation

    Article Title: The traditional Chinese patented medicine Qingke Pingchuan granules alleviate acute lung injury by regenerating club cells

    doi: 10.1002/pul2.12138

    Figure Lengend Snippet: Changes of inflammatory cytokine expression in the Control, LPS, and LPS + QKPCG groups. (a) Relative expression of IL‐6, IL‐1α, IL‐1β, TNF‐α, and TGF‐β mRNA. (b) The concentration of IL‐1α, IL‐1β, TNF‐α, and TGF‐β in BALF, as determined via ELISA. (c) IL‐6 concentration in BALF and plasma, as determined via ELISA. ns p > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. BALF, bronchoalveolar lavage fluid; ELISA, enzyme‐linked immunosorbent assay; LPS, lipopolysaccharide; QKPCG, Qingke Pingchuan granules; TGF‐β, transforming growth factor β; TNF‐α, tumor necrosis factor‐α.

    Article Snippet: The levels of inflammatory cytokines, including interleukin‐6 (IL‐6), IL‐1α, IL‐1β, tumor necrosis factor‐α (TNF‐α), and transforming growth factor β (TGF‐β) in the plasma and bronchoalveolar lavage fluid (BALF) were determined using respective ELISA kits—Mouse IL‐6 DuoSet ELISA (DY406; R&D), Mouse IL‐1 alpha/IL‐1F1 DuoSet ELISA (DY400; R&D), Mouse IL‐1 beta/IL‐1F2 DuoSet ELISA (DY401; R&D), Mouse TNF‐alpha DuoSet ELISA (DY410; R&D), and Mouse TGF‐beta 1 DuoSet ELISA (DY1679; R&D), as per the manufacturer's protocols.

    Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Changes in the expression of inflammatory cytokines following CC10 administration. (a) Concentration of IL‐6 in BALF detected via ELISA. (b) Concentration of TNF‐α in BALF detected via ELISA. (c) The concentration of IL‐1α in BALF detected via ELISA. (d) The concentration of IL‐1β in BALF detected via ELISA. * p < 0.05; ** p < 0.01; *** p < 0.001. BALF, bronchoalveolar lavage fluid; CC10, Clara cell 10; ELISA, enzyme‐linked immunosorbent assay; TNF‐α, tumor necrosis factor‐α.

    Journal: Pulmonary Circulation

    Article Title: The traditional Chinese patented medicine Qingke Pingchuan granules alleviate acute lung injury by regenerating club cells

    doi: 10.1002/pul2.12138

    Figure Lengend Snippet: Changes in the expression of inflammatory cytokines following CC10 administration. (a) Concentration of IL‐6 in BALF detected via ELISA. (b) Concentration of TNF‐α in BALF detected via ELISA. (c) The concentration of IL‐1α in BALF detected via ELISA. (d) The concentration of IL‐1β in BALF detected via ELISA. * p < 0.05; ** p < 0.01; *** p < 0.001. BALF, bronchoalveolar lavage fluid; CC10, Clara cell 10; ELISA, enzyme‐linked immunosorbent assay; TNF‐α, tumor necrosis factor‐α.

    Article Snippet: The levels of inflammatory cytokines, including interleukin‐6 (IL‐6), IL‐1α, IL‐1β, tumor necrosis factor‐α (TNF‐α), and transforming growth factor β (TGF‐β) in the plasma and bronchoalveolar lavage fluid (BALF) were determined using respective ELISA kits—Mouse IL‐6 DuoSet ELISA (DY406; R&D), Mouse IL‐1 alpha/IL‐1F1 DuoSet ELISA (DY400; R&D), Mouse IL‐1 beta/IL‐1F2 DuoSet ELISA (DY401; R&D), Mouse TNF‐alpha DuoSet ELISA (DY410; R&D), and Mouse TGF‐beta 1 DuoSet ELISA (DY1679; R&D), as per the manufacturer's protocols.

    Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Fig. 4. Effect of GGD treatment on lung in flammatory and ECM deposition in mice induced by BLM. On day 14 and 28 after BLM instillation, lung tissues of mice were separated and stained with H&E (A, red arrow: lymphocytes, black arrow: neutro phils) and Masson (B) staining (magnifica tion 200x), respectively. The content of hydroxyproline in the lung tissues was measured by ELISA (C). Data were expressed as means ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Journal of ethnopharmacology

    Article Title: Antifibrotic effect of Gancao Ganjiang decoction is mediated by PD-1 / TGF-β1 / IL-17A pathway in bleomycin-induced idiopathic pulmonary fibrosis.

    doi: 10.1016/j.jep.2021.114522

    Figure Lengend Snippet: Fig. 4. Effect of GGD treatment on lung in flammatory and ECM deposition in mice induced by BLM. On day 14 and 28 after BLM instillation, lung tissues of mice were separated and stained with H&E (A, red arrow: lymphocytes, black arrow: neutro phils) and Masson (B) staining (magnifica tion 200x), respectively. The content of hydroxyproline in the lung tissues was measured by ELISA (C). Data were expressed as means ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: The contents of hydroxyproline, IL-17A and TGF-β1 in lung tissues were measured using respective ELISA kits (CSB-E08839m, CSBE04608m, CSB-E04726m; Cusabio, China).

    Techniques: Staining, Enzyme-linked Immunosorbent Assay, Control

    Fig. 7. Effect of pirfenidone and GGD on the content of IL-17A in the lung tissues of BLM-induced mice. The content of IL-17A was analyzed by IHC staining (magnification 100x and 200x) (A) and ELISA (B). Data were expressed as mean ± SD, n = 6. ##P ＜ 0.01 vs. control group. **P ＜ 0.01, *P ＜ 0.05 vs. BLM.

    Journal: Journal of ethnopharmacology

    Article Title: Antifibrotic effect of Gancao Ganjiang decoction is mediated by PD-1 / TGF-β1 / IL-17A pathway in bleomycin-induced idiopathic pulmonary fibrosis.

    doi: 10.1016/j.jep.2021.114522

    Figure Lengend Snippet: Fig. 7. Effect of pirfenidone and GGD on the content of IL-17A in the lung tissues of BLM-induced mice. The content of IL-17A was analyzed by IHC staining (magnification 100x and 200x) (A) and ELISA (B). Data were expressed as mean ± SD, n = 6. ##P ＜ 0.01 vs. control group. **P ＜ 0.01, *P ＜ 0.05 vs. BLM.

    Article Snippet: The contents of hydroxyproline, IL-17A and TGF-β1 in lung tissues were measured using respective ELISA kits (CSB-E08839m, CSBE04608m, CSB-E04726m; Cusabio, China).

    Techniques: Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Control

    Fig. 8. Effect of pirfenidone and GGD on the content of TGF-β1 and mRNA/protein levels of EMT marker in the lung tissues. The content of TGF-β1 was detected by ELISA (A). The expression of E-cadherin, vimentin, and α-SMA was evaluated by qRT-PCR (B, C, D). The protein levels of E-cadherin, vimentin, and α-SMA were detected by Western blot (E). Data were expressed as mean ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM.

    Journal: Journal of ethnopharmacology

    Article Title: Antifibrotic effect of Gancao Ganjiang decoction is mediated by PD-1 / TGF-β1 / IL-17A pathway in bleomycin-induced idiopathic pulmonary fibrosis.

    doi: 10.1016/j.jep.2021.114522

    Figure Lengend Snippet: Fig. 8. Effect of pirfenidone and GGD on the content of TGF-β1 and mRNA/protein levels of EMT marker in the lung tissues. The content of TGF-β1 was detected by ELISA (A). The expression of E-cadherin, vimentin, and α-SMA was evaluated by qRT-PCR (B, C, D). The protein levels of E-cadherin, vimentin, and α-SMA were detected by Western blot (E). Data were expressed as mean ± SD, n = 6. ##P ＜ 0.01, #P ＜ 0.05 vs. control; **P ＜ 0.01, *P ＜ 0.05 vs. BLM.

    Article Snippet: The contents of hydroxyproline, IL-17A and TGF-β1 in lung tissues were measured using respective ELISA kits (CSB-E08839m, CSBE04608m, CSB-E04726m; Cusabio, China).

    Techniques: Marker, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot, Control